The Rams kept pushing for a tying goal, but could not find the shot they wanted, with things wrapping up at 1-0.
VCU out shot the Billikens 13-1 in the second half, with six shots on goal.
The Rams only scored once but that is all they needed to defeat cross-town rival University of Richmond.
A second half goal by junior Damian Gallegos gave VCU Men's Soccer (0-0-1) a 1-1 draw in its season opener against No. 23 New Hampshire.
A last-minute free kick by Dayton ended VCU's playoff dreams.
The two teams combined for a total of 5 shots on goal and only 19 shots overall.
A defensive battle ends with the Rams losing 1-0 on Tuesday night.
The Rams dominated much of the play, especially in the first half.
The Rams fell 4-1 to ODU on Tuesday night.
Neither team could find the back of the net in this 0-0 draw.